Improved lipid saponification for chromatographic quantification of fatty acids in porcine erythrocytes - an important lipidomic biomarker of the effectiveness of dietary fat supplementation in pigs as a large animal model for human studies
The objectives of our study were to enhance and evaluate an improved saponification method for the quantification of fatty acids (FA), with a specific focus on highly-unsaturated long-chain polyunsaturated FA (LPUFA) that are easily peroxidised in pig erythrocytes. These erythrocytes serve as a valuable large animal model for human studies. We implemented a modified saponification procedure, invol
