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Continuous measurements of a binding reaction using a capacitive biosensor

A capacitive biosensor with polyclonal antibodies raised against human serum albumin (HSA) immobilized on a gold transducer has been developed for continuous measurement of HSA in the μM-range. A mathematical model has been refined to describe integral HSA-binding curves assuming that (i) binding is essentially irreversible under the conditions used, (ii) the signal is scaled as the number of non-

A benchmark study of controlled emptying of equalization basins

Storm tanks, or equalization basins, have been used for many years in sewer or wastewater treatment systems to reduce the amount of combined sewer overflows. In this paper, a systematic control of an equalization basin is used not only to dampen or reduce the hydraulic load, but also to systematically improve the effluent quality in a nitrogen removal plant. It is demonstrated that the effluent am

Survival and long distance migration of brain-derived precursor cells transplanted to adult rat retina

Neural precursor cells transplanted to adult retina can integrate into the host. This is especially true when the neural precursor rat cell line RN33B is used. This cell line carries the reporter genes LacZ and green fluorescent protein (GFP). In grafted rat eyes, RN33B cells are localized from one eccentricity to the other of the host retina. In the present study, whole-mounted retinas were analy

Morphologies and droplet sizes of alkyd-acrylic hybrids with high solids content

Alkyd-acrylic hybrids having final solids content of 75-80% were prepared by dropping alkyd resin into an acrylic dispersion. The resulting suspo emulsion structures and the alkyd droplet sizes in the hybrids were dependent on the preparation procedure as well as the interfacial tension between the two liquid phases, sigma(12), the shear rate, gamma, the latex/alkyd ratio and the viscosity ratio,

Epitope spreading and autoimmune glomerulonephritis in rats induced by a T cell epitope of Goodpasture's antigen

An amino-terminal region of a, chain of type IV collagen noncollagenous domain [alpha(3)(IV)NC1] that induces experimental autoimmune glomerulortephritis (EAG) in rats has been identified. Only recombinant antigens that contain a nine-amino acid (AA) span of alpha(3)(IV)NC1, consistent with a T cell epitope, could induce EAG. It was hypothesized that synthetic peptides of this region should induce

Activated cell cycle checkpoints in epirubicin-treated breast cancer cells studied by BrdUrd-flow cytometry

Genetic alterations, such as p53 mutations, may affect a tumour's response to chemotherapy. We have treated two human breast cancer cell lines that differ in p53 status with epirubicin in order to study if there are differences in cell cycle kinetic response. MCF-7 cells express wild-type p53, while SK-BR-3 cells express only a mutated form of p53. The transition of cells from one cell cycle stage

Influence of the consumption of fatty Baltic Sea fish on plasma levels of halogenated environmental contaminants in Latvian and Swedish men

We examined the influence of widely varied consumption of fatty fish from the Baltic Sea and of age on plasma concentrations of polychlorinated biphenyls (PCBs), polychlorobiphenylols (OH-PCBs), 2, 2-bis(4-chlorophenyl)-1,1,1-trichloroethane (4,4'-DDT), 2, 2-bis(4-chlorophenyl)-1,1-dichloroethane (4,4'-DDE), 2,2',4, 4'-tetrabromodiphenyl ether (BDE-47), hexachlorobenzene (HCB), and pentachlorophen

Taxonomin i Svenska Akademiens grammatik. Några problem och lösningar

On various taxonomical problems and solutions in Svenska Akademiens grammatik (1999). Delimitiations of inflected and uninflected parts of speech. On the structure of prepositional phrases and unstressed verbs + stressed adverbial particles. Some new technical concepts: "satsbas" 'sentence basis', "annex" (for constituent which is loosely connected with a sentence).

Radiative lifetimes of Zr III excited levels

We report on radiative lifetimes of 4d5p excited states of Zr III produced in a laser produced plasma. The ions were populated either in the ground state or in metastable states, and the number of ions is strongly dependent on the application of an external magnetic field, which is shown to be very important when using the time-resolved laser-induced fluorescence technique for lifetime measurement

Application of environmental models to different hydrological systems

In recent years global problems such as climatic change, acid rain, and water pollution in surface and subsurface environments dominate discussions of world environmental problems. In this paper, the roles of hydrologic processes and hydrogeochemical processes are investigated through development, modification, and application of mathematical models for addressing point and non-point source water